ssr_primer
Primer Details for TRINITY_DN64102_c5_g1_i3 |
|---|
| (T)20: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGGATGAAAGTCGGCAACA | TGTCAAGCACGGCAAAACTG | Start: 477 |
| Tm | 59.966 | 59.9 | End: 603 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | TCGGATGAAAGTCGGCAACA | CCTTGTCAAGCACGGCAAAA | Start: 477 |
| Tm | 59.966 | 59.898 | End: 606 |
| Size | 20 | 20 | 130 |
| Primer 3 details |
|---|
| Sequence | CGGATGAAAGTCGGCAACAC | TGTCAAGCACGGCAAAACTG | Start: 478 |
| Tm | 59.834 | 59.9 | End: 603 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
|---|
| Sequence | CGGATGAAAGTCGGCAACAC | CCTTGTCAAGCACGGCAAAA | Start: 478 |
| Tm | 59.834 | 59.898 | End: 606 |
| Size | 20 | 20 | 129 |
| Primer 5 details |
|---|
| Sequence | GAGGTGGCAGTGTGGACATT | GCTACGGCGCAACTAATTGG | Start: 714 |
| Tm | 60.251 | 59.972 | End: 887 |
| Size | 20 | 20 | 174 |
(T)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGGATGAAAGTCGGCAACA | TGTCAAGCACGGCAAAACTG | Start: 477 |
| Tm | 59.966 | 59.9 | End: 603 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | TCGGATGAAAGTCGGCAACA | CCTTGTCAAGCACGGCAAAA | Start: 477 |
| Tm | 59.966 | 59.898 | End: 606 |
| Size | 20 | 20 | 130 |
| Primer 3 details |
|---|
| Sequence | CGGATGAAAGTCGGCAACAC | TGTCAAGCACGGCAAAACTG | Start: 478 |
| Tm | 59.834 | 59.9 | End: 603 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
|---|
| Sequence | CGGATGAAAGTCGGCAACAC | CCTTGTCAAGCACGGCAAAA | Start: 478 |
| Tm | 59.834 | 59.898 | End: 606 |
| Size | 20 | 20 | 129 |
| Primer 5 details |
|---|
| Sequence | GAGGTGGCAGTGTGGACATT | GCTACGGCGCAACTAATTGG | Start: 714 |
| Tm | 60.251 | 59.972 | End: 887 |
| Size | 20 | 20 | 174 |
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