ssr_primer
Primer Details for TRINITY_DN64058_c6_g3_i1 |
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| (A)13(ATAAATAAATAAATAAATTCAAGCTACACAAAAATATTAATTTTTACACACGGGAGAGGGGTGGCACCAAAGTAGATCATTGAACGAAGTAAGTAAATTGAGCACCATGAGACTTGCTTGCTCTTTAAGTTGGAGGTGTTTTCAGAGAATATGATAGGAACATTGTAAGTTTTTGCAATTTAGCTCGACTTGAGAAATTAACATTATCTATGTCCGTCAATTCAAATATCCTGCTTGTTGAGACAAAAGTCGACCCAAGTCTTTAAGCCGTGATTACTTTTTATTTGGCTTACTTGGACTCTAAGCTGCAGAAATTTCCCAGTCATACATCATGATCACAAGACCACTCACAAGAAAAACTTTTGGTGAATTTCGCATCTTTTTACAGAGCAATTTGAGTGGGGTTGGTGTTGCGTGCATTAAAGACAAAAAAAGGCGATAGCTTGGACAATTTTTAGGGAAAAGATGTCTTTAAAACGGGAATGCCGTTTCGATAAATGAGACGGCTCAGAACTTTTTGCACCCCTGCTCGGATCTCTTATGCTGGATCCAGAGGTTTAAATTGATCTTTTTCAATGTGGACTGAAACATTTAAGTAGTTTCTTGCCAAAGATGGTATAAAATGCGGACAAGATACTCTCGATTTATAATTTTTTCATCCTT)(AAAT)4: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTGTGACGATCATGCAAGC | ACTGAAGTCTGGTGTGCTGG | Start: 707 |
| Tm | 60.248 | 59.893 | End: 935 |
| Size | 20 | 20 | 229 |
| Primer 2 details |
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| Sequence | TTGATCAGTCGACTGCGCAT | GCTTGCATGATCGTCACAGC | Start: 614 |
| Tm | 60.109 | 60.248 | End: 726 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
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| Sequence | ACTTTCGAGACACTGGCCAA | CCATGCTGCAATCATACGCC | Start: 1030 |
| Tm | 59.532 | 60.04 | End: 1198 |
| Size | 20 | 20 | 169 |
| Primer 4 details |
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| Sequence | CCAGCACACCAGACTTCAGT | GCTGCAATCATACGCCAACA | Start: 916 |
| Tm | 59.893 | 59.549 | End: 1194 |
| Size | 20 | 20 | 279 |
| Primer 5 details |
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| Sequence | CCAGCACACCAGACTTCAGT | TGCTGCAATCATACGCCAAC | Start: 916 |
| Tm | 59.893 | 59.549 | End: 1195 |
| Size | 20 | 20 | 280 |
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