Primer Details for TRINITY_DN63929_c9_g1_i1 | |||
|---|---|---|---|
| (A)14: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACATTTAGAGCTGTTTCACCAACG | TGCATGATTTGTGGAAAACATTGA | Start: 95 |
| Tm | 60.024 | 58.392 | End: 195 |
| Size | 24 | 24 | 101 |
| Primer 2 details | |||
| Sequence | CATTTAGAGCTGTTTCACCAACGA | TGCATGATTTGTGGAAAACATTGA | Start: 96 |
| Tm | 59.787 | 58.392 | End: 195 |
| Size | 24 | 24 | 100 |
| Primer 3 details | |||
| Sequence | AGAGCTGTTTCACCAACGAT | AAAAATGCATGATTTGTGGAAAACA | Start: 101 |
| Tm | 57.449 | 57.669 | End: 200 |
| Size | 20 | 25 | 100 |
| Primer 4 details | |||
| Sequence | ACATTTAGAGCTGTTTCACCAACG | TGCATGATTTGTGGAAAACATTG | Start: 95 |
| Tm | 60.024 | 57.074 | End: 195 |
| Size | 24 | 23 | 101 |
| Primer 5 details | |||
| Sequence | CATTTAGAGCTGTTTCACCAACG | TGCATGATTTGTGGAAAACATTGA | Start: 96 |
| Tm | 58.533 | 58.392 | End: 195 |
| Size | 23 | 24 | 100 |
