Primer Details for TRINITY_DN63920_c9_g1_i1 | |||
|---|---|---|---|
| (T)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAAATTGATGTCCGATTCTTTGT | ACTACACGGGACCGGAAATT | Start: 24 |
| Tm | 59.523 | 59.021 | End: 136 |
| Size | 25 | 20 | 113 |
| Primer 2 details | |||
| Sequence | TGTCCGATTCTTTGTTTAGGATGT | ACTACACGGGACCGGAAATT | Start: 34 |
| Tm | 58.443 | 59.021 | End: 136 |
| Size | 24 | 20 | 103 |
| Primer 3 details | |||
| Sequence | TGTCCGATTCTTTGTTTAGGATGTC | ACTACACGGGACCGGAAATT | Start: 34 |
| Tm | 59.358 | 59.021 | End: 136 |
| Size | 25 | 20 | 103 |
| Primer 4 details | |||
| Sequence | CCAAATTGATGTCCGATTCTTTGT | ACTACACGGGACCGGAAATT | Start: 25 |
| Tm | 58.287 | 59.021 | End: 136 |
| Size | 24 | 20 | 112 |
| Primer 5 details | |||
| Sequence | TGATGTCCGATTCTTTGTTTAGGA | ACTACACGGGACCGGAAATT | Start: 31 |
| Tm | 58.2 | 59.021 | End: 136 |
| Size | 24 | 20 | 106 |
