ssr_primer
Primer Details for TRINITY_DN63870_c2_g1_i2 |
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| (T)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CATCGGTTTTTGGAGCGCAA | TCATGAGTCTGGTTCACGGC | Start: 460 |
| Tm | 60.04 | 60.037 | End: 727 |
| Size | 20 | 20 | 268 |
| Primer 2 details |
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| Sequence | GCTGTCAGCTTGTTGAACCG | TCATGAGTCTGGTTCACGGC | Start: 584 |
| Tm | 60.041 | 60.037 | End: 727 |
| Size | 20 | 20 | 144 |
| Primer 3 details |
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| Sequence | CATCGGTTTTTGGAGCGCAA | CGGTTCAACAAGCTGACAGC | Start: 460 |
| Tm | 60.04 | 60.041 | End: 603 |
| Size | 20 | 20 | 144 |
| Primer 4 details |
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| Sequence | TGGAGCGCAATACAGGATCC | TCATGAGTCTGGTTCACGGC | Start: 470 |
| Tm | 59.893 | 60.037 | End: 727 |
| Size | 20 | 20 | 258 |
| Primer 5 details |
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| Sequence | TGGAGCGCAATACAGGATCC | CGGTTCAACAAGCTGACAGC | Start: 470 |
| Tm | 59.893 | 60.041 | End: 603 |
| Size | 20 | 20 | 134 |
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