Primer Details for TRINITY_DN63841_c2_g1_i1 |
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| (T)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACCAATCGGATCAGCACCT | ACATTGTGTCGAAAGAATTTTCTTGA | Start: 68 |
| Tm | 59.821 | 58.52 | End: 179 |
| Size | 20 | 26 | 112 |
| Primer 2 details |
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| Sequence | GACCAATCGGATCAGCACCT | GACATTGTGTCGAAAGAATTTTCTTGA | Start: 68 |
| Tm | 59.821 | 59.356 | End: 180 |
| Size | 20 | 27 | 113 |
| Primer 3 details |
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| Sequence | GACCAATCGGATCAGCACCT | ACATTGTGTCGAAAGAATTTTCTTG | Start: 68 |
| Tm | 59.821 | 57.316 | End: 179 |
| Size | 20 | 25 | 112 |
| Primer 4 details |
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| Sequence | GACCAATCGGATCAGCACCT | ACCTCCAAATATTTAAACACGATGT | Start: 68 |
| Tm | 59.821 | 57.303 | End: 222 |
| Size | 20 | 25 | 155 |
| Primer 5 details |
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| Sequence | GACCAATCGGATCAGCACCT | GACATTGTGTCGAAAGAATTTTCTTG | Start: 68 |
| Tm | 59.821 | 58.232 | End: 180 |
| Size | 20 | 26 | 113 |
