ssr_primer
Primer Details for TRINITY_DN63747_c6_g1_i9 |
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| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGGTCGACCAGGAATGAAT | CTTTTATCGCGGGTCTGGGT | Start: 686 |
| Tm | 59.822 | 60.108 | End: 830 |
| Size | 20 | 20 | 145 |
| Primer 2 details |
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| Sequence | CCGGTCGACCAGGAATGAAT | TTTTATCGCGGGTCTGGGTC | Start: 686 |
| Tm | 59.822 | 60.108 | End: 829 |
| Size | 20 | 20 | 144 |
| Primer 3 details |
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| Sequence | GTGCAAGAGCGGAAGAGAGA | CTTTTATCGCGGGTCTGGGT | Start: 625 |
| Tm | 59.755 | 60.108 | End: 830 |
| Size | 20 | 20 | 206 |
| Primer 4 details |
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| Sequence | GTGCAAGAGCGGAAGAGAGA | TTTTATCGCGGGTCTGGGTC | Start: 625 |
| Tm | 59.755 | 60.108 | End: 829 |
| Size | 20 | 20 | 205 |
| Primer 5 details |
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| Sequence | AGCAGGTTGGTGTCGATAGC | TCTCTCTTCCGCTCTTGCAC | Start: 424 |
| Tm | 60.108 | 59.755 | End: 644 |
| Size | 20 | 20 | 221 |
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