ssr_primer
Primer Details for TRINITY_DN63731_c2_g1_i1 |
|---|
| (T)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGTTGGGCAAGTTATCCGG | AACAGCCCCCAAAAGGTCAA | Start: 653 |
| Tm | 60.034 | 60.032 | End: 903 |
| Size | 20 | 20 | 251 |
| Primer 2 details |
|---|
| Sequence | CCTCGCACATGGAAAGACCT | CCGTATCGGGCAAGAGAGTC | Start: 238 |
| Tm | 60.036 | 59.969 | End: 434 |
| Size | 20 | 20 | 197 |
| Primer 3 details |
|---|
| Sequence | TCCTCGCACATGGAAAGACC | CCGTATCGGGCAAGAGAGTC | Start: 237 |
| Tm | 60.036 | 59.969 | End: 434 |
| Size | 20 | 20 | 198 |
| Primer 4 details |
|---|
| Sequence | CCACGCTCTGGCTTTCTACA | CCGTATCGGGCAAGAGAGTC | Start: 333 |
| Tm | 60.037 | 59.969 | End: 434 |
| Size | 20 | 20 | 102 |
| Primer 5 details |
|---|
| Sequence | AGGTTGGGCAAGTTATCCGG | AGGGTCTGCTGTTTCGACAG | Start: 653 |
| Tm | 60.034 | 59.966 | End: 861 |
| Size | 20 | 20 | 209 |
(T)17gaatctccttctaaattttttttagattttgattggtacctaggtataattgtaatagttgctatcctaaatttcgtttatgcttaa(T)24: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGTTGGGCAAGTTATCCGG | AACAGCCCCCAAAAGGTCAA | Start: 653 |
| Tm | 60.034 | 60.032 | End: 903 |
| Size | 20 | 20 | 251 |
| Primer 2 details |
|---|
| Sequence | CCTCGCACATGGAAAGACCT | CCGTATCGGGCAAGAGAGTC | Start: 238 |
| Tm | 60.036 | 59.969 | End: 434 |
| Size | 20 | 20 | 197 |
| Primer 3 details |
|---|
| Sequence | TCCTCGCACATGGAAAGACC | CCGTATCGGGCAAGAGAGTC | Start: 237 |
| Tm | 60.036 | 59.969 | End: 434 |
| Size | 20 | 20 | 198 |
| Primer 4 details |
|---|
| Sequence | CCACGCTCTGGCTTTCTACA | CCGTATCGGGCAAGAGAGTC | Start: 333 |
| Tm | 60.037 | 59.969 | End: 434 |
| Size | 20 | 20 | 102 |
| Primer 5 details |
|---|
| Sequence | AGGTTGGGCAAGTTATCCGG | AGGGTCTGCTGTTTCGACAG | Start: 653 |
| Tm | 60.034 | 59.966 | End: 861 |
| Size | 20 | 20 | 209 |
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