ssr_primer
Primer Details for TRINITY_DN63714_c4_g1_i2 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGCAGTTCCGATACCTCT | CGTGGTCTTGTCGACTCACA | Start: 3221 |
| Tm | 60.032 | 59.97 | End: 3447 |
| Size | 20 | 20 | 227 |
| Primer 2 details |
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| Sequence | AGGGCAGTTCCGATACCTCT | CACGTGGTCTTGTCGACTCA | Start: 3221 |
| Tm | 60.032 | 59.97 | End: 3449 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
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| Sequence | AGGGCAGTTCCGATACCTCT | ACGTGGTCTTGTCGACTCAC | Start: 3221 |
| Tm | 60.032 | 59.97 | End: 3448 |
| Size | 20 | 20 | 228 |
| Primer 4 details |
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| Sequence | CCTCAGGAGAATGCCAAGCA | AGAGGTAGATGCCGGACAGT | Start: 510 |
| Tm | 60.035 | 60.033 | End: 735 |
| Size | 20 | 20 | 226 |
| Primer 5 details |
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| Sequence | CCTTCATGTTCGTCGGGTCA | AGAGGTAGATGCCGGACAGT | Start: 476 |
| Tm | 60.038 | 60.033 | End: 735 |
| Size | 20 | 20 | 260 |
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