ssr_primer
Primer Details for TRINITY_DN63710_c10_g2_i1 |
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| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACAAACCCGGAACCAGTGT | ACGGGGCAGAGAAACCTTTT | Start: 8 |
| Tm | 60.034 | 59.815 | End: 245 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
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| Sequence | CTGCCAGAAACGTCCGTTTG | ACGGGGCAGAGAAACCTTTT | Start: 72 |
| Tm | 60.041 | 59.815 | End: 245 |
| Size | 20 | 20 | 174 |
| Primer 3 details |
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| Sequence | TACGAGCTTTGAGTCCTGCC | TCCATTAGTGTTCGCCGCTT | Start: 868 |
| Tm | 59.753 | 60.037 | End: 1107 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
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| Sequence | TACGAGCTTTGAGTCCTGCC | TTCCATTAGTGTTCGCCGCT | Start: 868 |
| Tm | 59.753 | 60.037 | End: 1108 |
| Size | 20 | 20 | 241 |
| Primer 5 details |
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| Sequence | AACAAACCCGGAACCAGTGT | GACGGGGCAGAGAAACCTTT | Start: 8 |
| Tm | 60.034 | 60.251 | End: 246 |
| Size | 20 | 20 | 239 |
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