ssr_primer
Primer Details for TRINITY_DN63658_c0_g1_i2 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACCACAAAATCGCGGTTT | AAACGGTCAGCTGGTCAGAG | Start: 232 |
| Tm | 59.969 | 59.966 | End: 403 |
| Size | 20 | 20 | 172 |
| Primer 2 details |
|---|
| Sequence | CCACCACAAAATCGCGGTTT | GGTTGTTTCACGCCGTTCAA | Start: 232 |
| Tm | 59.969 | 59.901 | End: 372 |
| Size | 20 | 20 | 141 |
| Primer 3 details |
|---|
| Sequence | CCACCACAAAATCGCGGTTT | TTGGTTGTTTCACGCCGTTC | Start: 232 |
| Tm | 59.969 | 59.901 | End: 374 |
| Size | 20 | 20 | 143 |
| Primer 4 details |
|---|
| Sequence | CCCCTGCTGCTTAGTGGTAC | AAACGGTCAGCTGGTCAGAG | Start: 203 |
| Tm | 60.108 | 59.966 | End: 403 |
| Size | 20 | 20 | 201 |
| Primer 5 details |
|---|
| Sequence | TCCCCCTACCCCCAGAAAAT | AAACGGTCAGCTGGTCAGAG | Start: 138 |
| Tm | 59.875 | 59.966 | End: 403 |
| Size | 20 | 20 | 266 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACCACAAAATCGCGGTTT | AAACGGTCAGCTGGTCAGAG | Start: 232 |
| Tm | 59.969 | 59.966 | End: 403 |
| Size | 20 | 20 | 172 |
| Primer 2 details |
|---|
| Sequence | CCACCACAAAATCGCGGTTT | GGTTGTTTCACGCCGTTCAA | Start: 232 |
| Tm | 59.969 | 59.901 | End: 372 |
| Size | 20 | 20 | 141 |
| Primer 3 details |
|---|
| Sequence | CCACCACAAAATCGCGGTTT | TTGGTTGTTTCACGCCGTTC | Start: 232 |
| Tm | 59.969 | 59.901 | End: 374 |
| Size | 20 | 20 | 143 |
| Primer 4 details |
|---|
| Sequence | CCCCTGCTGCTTAGTGGTAC | AAACGGTCAGCTGGTCAGAG | Start: 203 |
| Tm | 60.108 | 59.966 | End: 403 |
| Size | 20 | 20 | 201 |
| Primer 5 details |
|---|
| Sequence | TCCCCCTACCCCCAGAAAAT | AAACGGTCAGCTGGTCAGAG | Start: 138 |
| Tm | 59.875 | 59.966 | End: 403 |
| Size | 20 | 20 | 266 |
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