ssr_primer
Primer Details for TRINITY_DN63591_c1_g2_i4 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCATTCATGGGGGCTTGACT | CGGCGGAACTGGTTTTAAGC | Start: 141 |
| Tm | 60.033 | 60.11 | End: 405 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
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| Sequence | TTTTCATGCGCTTTTCGGGG | TCCCGTTTTGAGCGAATTGC | Start: 969 |
| Tm | 60.039 | 59.76 | End: 1238 |
| Size | 20 | 20 | 270 |
| Primer 3 details |
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| Sequence | TTTTTGATCATGCCACCGCC | CCCCGAAAAGCGCATGAAAA | Start: 766 |
| Tm | 59.754 | 60.039 | End: 988 |
| Size | 20 | 20 | 223 |
| Primer 4 details |
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| Sequence | GCTTAAAACCAGTTCCGCCG | CCCCCGTCGGAGTATTAGGA | Start: 386 |
| Tm | 60.11 | 60.179 | End: 486 |
| Size | 20 | 20 | 101 |
| Primer 5 details |
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| Sequence | TGCCACCGCCAGAACAAATA | CCCCGAAAAGCGCATGAAAA | Start: 776 |
| Tm | 60.251 | 60.039 | End: 988 |
| Size | 20 | 20 | 213 |
(G)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCATTCATGGGGGCTTGACT | CGGCGGAACTGGTTTTAAGC | Start: 141 |
| Tm | 60.033 | 60.11 | End: 405 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
|---|
| Sequence | TTTTCATGCGCTTTTCGGGG | TCCCGTTTTGAGCGAATTGC | Start: 969 |
| Tm | 60.039 | 59.76 | End: 1238 |
| Size | 20 | 20 | 270 |
| Primer 3 details |
|---|
| Sequence | TTTTTGATCATGCCACCGCC | CCCCGAAAAGCGCATGAAAA | Start: 766 |
| Tm | 59.754 | 60.039 | End: 988 |
| Size | 20 | 20 | 223 |
| Primer 4 details |
|---|
| Sequence | GCTTAAAACCAGTTCCGCCG | CCCCCGTCGGAGTATTAGGA | Start: 386 |
| Tm | 60.11 | 60.179 | End: 486 |
| Size | 20 | 20 | 101 |
| Primer 5 details |
|---|
| Sequence | TGCCACCGCCAGAACAAATA | CCCCGAAAAGCGCATGAAAA | Start: 776 |
| Tm | 60.251 | 60.039 | End: 988 |
| Size | 20 | 20 | 213 |
(T)22: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCATTCATGGGGGCTTGACT | CGGCGGAACTGGTTTTAAGC | Start: 141 |
| Tm | 60.033 | 60.11 | End: 405 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
|---|
| Sequence | TTTTCATGCGCTTTTCGGGG | TCCCGTTTTGAGCGAATTGC | Start: 969 |
| Tm | 60.039 | 59.76 | End: 1238 |
| Size | 20 | 20 | 270 |
| Primer 3 details |
|---|
| Sequence | TTTTTGATCATGCCACCGCC | CCCCGAAAAGCGCATGAAAA | Start: 766 |
| Tm | 59.754 | 60.039 | End: 988 |
| Size | 20 | 20 | 223 |
| Primer 4 details |
|---|
| Sequence | GCTTAAAACCAGTTCCGCCG | CCCCCGTCGGAGTATTAGGA | Start: 386 |
| Tm | 60.11 | 60.179 | End: 486 |
| Size | 20 | 20 | 101 |
| Primer 5 details |
|---|
| Sequence | TGCCACCGCCAGAACAAATA | CCCCGAAAAGCGCATGAAAA | Start: 776 |
| Tm | 60.251 | 60.039 | End: 988 |
| Size | 20 | 20 | 213 |
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