ssr_primer
Primer Details for TRINITY_DN63540_c1_g2_i1 |
|---|
| (T)31: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCGGATCTGACACGGAAA | CCCCATGACCCACTGAGTTC | Start: 3501 |
| Tm | 59.966 | 60.035 | End: 3642 |
| Size | 20 | 20 | 142 |
| Primer 2 details |
|---|
| Sequence | TTGCGGATCTGACACGGAAA | GTTCCCCATGACCCACTGAG | Start: 3501 |
| Tm | 59.966 | 60.035 | End: 3645 |
| Size | 20 | 20 | 145 |
| Primer 3 details |
|---|
| Sequence | TTTGCGGATCTGACACGGAA | CCCCATGACCCACTGAGTTC | Start: 3500 |
| Tm | 59.966 | 60.035 | End: 3642 |
| Size | 20 | 20 | 143 |
| Primer 4 details |
|---|
| Sequence | TTTGCGGATCTGACACGGAA | GTTCCCCATGACCCACTGAG | Start: 3500 |
| Tm | 59.966 | 60.035 | End: 3645 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
|---|
| Sequence | TTGCGGATCTGACACGGAAA | TGGATTGTGAAGGAGACCGC | Start: 3501 |
| Tm | 59.966 | 60.036 | End: 3715 |
| Size | 20 | 20 | 215 |
(AAAG)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCGGATCTGACACGGAAA | CCCCATGACCCACTGAGTTC | Start: 3501 |
| Tm | 59.966 | 60.035 | End: 3642 |
| Size | 20 | 20 | 142 |
| Primer 2 details |
|---|
| Sequence | TTGCGGATCTGACACGGAAA | GTTCCCCATGACCCACTGAG | Start: 3501 |
| Tm | 59.966 | 60.035 | End: 3645 |
| Size | 20 | 20 | 145 |
| Primer 3 details |
|---|
| Sequence | TTTGCGGATCTGACACGGAA | CCCCATGACCCACTGAGTTC | Start: 3500 |
| Tm | 59.966 | 60.035 | End: 3642 |
| Size | 20 | 20 | 143 |
| Primer 4 details |
|---|
| Sequence | TTTGCGGATCTGACACGGAA | GTTCCCCATGACCCACTGAG | Start: 3500 |
| Tm | 59.966 | 60.035 | End: 3645 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
|---|
| Sequence | TTGCGGATCTGACACGGAAA | TGGATTGTGAAGGAGACCGC | Start: 3501 |
| Tm | 59.966 | 60.036 | End: 3715 |
| Size | 20 | 20 | 215 |
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