ssr_primer
Primer Details for TRINITY_DN63498_c0_g2_i3 |
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| (T)10gtgaccccaaaaaaagagctgagagtcaaaaaac(A)32tcgtccttgtgaacatgaaaagttgcggatacaactataaaattcgataccttatataatagacacacca(C)13(A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGAACATGAAAAGTTGCGGA | GGGCGGGTACATTTACTTCTCA | Start: 122 |
| Tm | 59.315 | 60.093 | End: 259 |
| Size | 22 | 22 | 138 |
| Primer 2 details |
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| Sequence | TGTGAACATGAAAAGTTGCGGA | AGGGCGGGTACATTTACTTCTC | Start: 122 |
| Tm | 59.315 | 59.83 | End: 260 |
| Size | 22 | 22 | 139 |
| Primer 3 details |
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| Sequence | TGTGAACATGAAAAGTTGCGGA | AGGGCGGGTACATTTACTTCT | Start: 122 |
| Tm | 59.315 | 58.806 | End: 260 |
| Size | 22 | 21 | 139 |
| Primer 4 details |
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| Sequence | GCAAATTTCAGCCTTTTTCCCC | GGGCGGGTACATTTACTTCTCA | Start: 10 |
| Tm | 59.186 | 60.093 | End: 259 |
| Size | 22 | 22 | 250 |
| Primer 5 details |
|---|
| Sequence | GCAAATTTCAGCCTTTTTCCCC | AGGGCGGGTACATTTACTTCTC | Start: 10 |
| Tm | 59.186 | 59.83 | End: 260 |
| Size | 22 | 22 | 251 |
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