Primer Details for TRINITY_DN63455_c4_g6_i1 | |||
|---|---|---|---|
| (A)23: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCCTCCTAACAACAGAATTGC | GTCAAAACGACATGCGATATACAGT | Start: 99 |
| Tm | 58.915 | 59.995 | End: 198 |
| Size | 22 | 25 | 100 |
| Primer 2 details | |||
| Sequence | AGCCTCCTAACAACAGAATTGC | GTCAAAACGACATGCGATATACA | Start: 99 |
| Tm | 58.915 | 57.616 | End: 198 |
| Size | 22 | 23 | 100 |
| Primer 3 details | |||
| Sequence | AGCCTCCTAACAACAGAATTGC | GTCAAAACGACATGCGATATACAG | Start: 99 |
| Tm | 58.915 | 58.576 | End: 198 |
| Size | 22 | 24 | 100 |
| Primer 4 details | |||
| Sequence | TCGATTGCGATATTTTGTCTCAAA | CGCAATTCTGTTGTTAGGAGGC | Start: 3 |
| Tm | 57.584 | 60.16 | End: 121 |
| Size | 24 | 22 | 119 |
| Primer 5 details | |||
| Sequence | TCGATTGCGATATTTTGTCTCAAAA | CGCAATTCTGTTGTTAGGAGGC | Start: 3 |
| Tm | 58.163 | 60.16 | End: 121 |
| Size | 25 | 22 | 119 |
