ssr_primer
Primer Details for TRINITY_DN63422_c4_g2_i4 |
|---|
| (T)19: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGCGTTGGACAAGAAAACC | ACGAGGGTGTCTAGCAAAGC | Start: 2219 |
| Tm | 59.968 | 60.037 | End: 2322 |
| Size | 20 | 20 | 104 |
| Primer 2 details |
|---|
| Sequence | GGGCGTTGGACAAGAAAACC | CGAGGGTGTCTAGCAAAGCA | Start: 2219 |
| Tm | 59.968 | 60.037 | End: 2321 |
| Size | 20 | 20 | 103 |
| Primer 3 details |
|---|
| Sequence | ACACTTACCATCGCCTGCAA | TTGGTGACTATCGTTGGCCC | Start: 1375 |
| Tm | 59.964 | 60.036 | End: 1614 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
|---|
| Sequence | TCGCCTGCAAGCACTTGATA | TTGGTGACTATCGTTGGCCC | Start: 1385 |
| Tm | 60.036 | 60.036 | End: 1614 |
| Size | 20 | 20 | 230 |
| Primer 5 details |
|---|
| Sequence | ATGTGCCGTGATTGAATGCG | GGTTTTCTTGTCCAACGCCC | Start: 1993 |
| Tm | 59.901 | 59.968 | End: 2238 |
| Size | 20 | 20 | 246 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGCGTTGGACAAGAAAACC | ACGAGGGTGTCTAGCAAAGC | Start: 2219 |
| Tm | 59.968 | 60.037 | End: 2322 |
| Size | 20 | 20 | 104 |
| Primer 2 details |
|---|
| Sequence | GGGCGTTGGACAAGAAAACC | CGAGGGTGTCTAGCAAAGCA | Start: 2219 |
| Tm | 59.968 | 60.037 | End: 2321 |
| Size | 20 | 20 | 103 |
| Primer 3 details |
|---|
| Sequence | ACACTTACCATCGCCTGCAA | TTGGTGACTATCGTTGGCCC | Start: 1375 |
| Tm | 59.964 | 60.036 | End: 1614 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
|---|
| Sequence | TCGCCTGCAAGCACTTGATA | TTGGTGACTATCGTTGGCCC | Start: 1385 |
| Tm | 60.036 | 60.036 | End: 1614 |
| Size | 20 | 20 | 230 |
| Primer 5 details |
|---|
| Sequence | ATGTGCCGTGATTGAATGCG | GGTTTTCTTGTCCAACGCCC | Start: 1993 |
| Tm | 59.901 | 59.968 | End: 2238 |
| Size | 20 | 20 | 246 |
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