Primer Details for TRINITY_DN63396_c2_g1_i1 | |||
|---|---|---|---|
| (T)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAACTTTCCGTTGTTTGAATGTTT | CCGTATGAACCTTGAGGCGT | Start: 5 |
| Tm | 57.598 | 60.108 | End: 203 |
| Size | 24 | 20 | 199 |
| Primer 2 details | |||
| Sequence | CAACTTTCCGTTGTTTGAATGTTT | ACGCCGTATGAACCTTGAGG | Start: 5 |
| Tm | 57.598 | 60.108 | End: 206 |
| Size | 24 | 20 | 202 |
| Primer 3 details | |||
| Sequence | CAACTTTCCGTTGTTTGAATGTTT | ATGAACCTTGAGGCGTTGCT | Start: 5 |
| Tm | 57.598 | 60.251 | End: 199 |
| Size | 24 | 20 | 195 |
| Primer 4 details | |||
| Sequence | CAACTTTCCGTTGTTTGAATGTTT | TGAACCTTGAGGCGTTGCTA | Start: 5 |
| Tm | 57.598 | 59.605 | End: 198 |
| Size | 24 | 20 | 194 |
| Primer 5 details | |||
| Sequence | CAACTTTCCGTTGTTTGAATGTTTT | CCGTATGAACCTTGAGGCGT | Start: 5 |
| Tm | 58.173 | 60.108 | End: 203 |
| Size | 25 | 20 | 199 |
