ssr_primer
Primer Details for TRINITY_DN63383_c5_g1_i5 |
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| (T)14ctgttaggggtggaaacctgactgatccagtgctctgaagtttgagaataagcttttatttaatttgata(T)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGTCGAACCCCACAGGATG | GGGCCCATTGTGTGAGATGA | Start: 3 |
| Tm | 60.108 | 60.034 | End: 271 |
| Size | 20 | 20 | 269 |
| Primer 2 details |
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| Sequence | TCATCTCACACAATGGGCCC | CGAACAGGTTGGGGAAGGAA | Start: 252 |
| Tm | 60.034 | 59.89 | End: 371 |
| Size | 20 | 20 | 120 |
| Primer 3 details |
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| Sequence | CAACACGGGTCATGGCATTC | GGGCCCATTGTGTGAGATGA | Start: 36 |
| Tm | 59.829 | 60.034 | End: 271 |
| Size | 20 | 20 | 236 |
| Primer 4 details |
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| Sequence | TCATCTCACACAATGGGCCC | ACAGGTTGGGGAAGGAAACG | Start: 252 |
| Tm | 60.034 | 60.179 | End: 368 |
| Size | 20 | 20 | 117 |
| Primer 5 details |
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| Sequence | AGGCACTCACCAGTCAACAC | GGGCCCATTGTGTGAGATGA | Start: 124 |
| Tm | 60.179 | 60.034 | End: 271 |
| Size | 20 | 20 | 148 |
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