ssr_primer
Primer Details for TRINITY_DN63309_c1_g1_i18 |
|---|
| (GAA)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAGAAACACAGGCAGTTGC | TGCCCTGCCATCATCCAATT | Start: 3162 |
| Tm | 59.969 | 60.032 | End: 3262 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | TTTCCCGCGAACGTAAAGGA | TGCCCTGCCATCATCCAATT | Start: 3107 |
| Tm | 59.967 | 60.032 | End: 3262 |
| Size | 20 | 20 | 156 |
| Primer 3 details |
|---|
| Sequence | AATTGGATGATGGCAGGGCA | CCGTGAAGTACTGTGACGCT | Start: 3243 |
| Tm | 60.032 | 60.039 | End: 3490 |
| Size | 20 | 20 | 248 |
| Primer 4 details |
|---|
| Sequence | CCAGAAACACAGGCAGTTGC | CCCCAACTCCCTCTCCCTAA | Start: 3162 |
| Tm | 59.969 | 59.956 | End: 3424 |
| Size | 20 | 20 | 263 |
| Primer 5 details |
|---|
| Sequence | AATTGGATGATGGCAGGGCA | CCCCAACTCCCTCTCCCTAA | Start: 3243 |
| Tm | 60.032 | 59.956 | End: 3424 |
| Size | 20 | 20 | 182 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAGAAACACAGGCAGTTGC | TGCCCTGCCATCATCCAATT | Start: 3162 |
| Tm | 59.969 | 60.032 | End: 3262 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | TTTCCCGCGAACGTAAAGGA | TGCCCTGCCATCATCCAATT | Start: 3107 |
| Tm | 59.967 | 60.032 | End: 3262 |
| Size | 20 | 20 | 156 |
| Primer 3 details |
|---|
| Sequence | AATTGGATGATGGCAGGGCA | CCGTGAAGTACTGTGACGCT | Start: 3243 |
| Tm | 60.032 | 60.039 | End: 3490 |
| Size | 20 | 20 | 248 |
| Primer 4 details |
|---|
| Sequence | CCAGAAACACAGGCAGTTGC | CCCCAACTCCCTCTCCCTAA | Start: 3162 |
| Tm | 59.969 | 59.956 | End: 3424 |
| Size | 20 | 20 | 263 |
| Primer 5 details |
|---|
| Sequence | AATTGGATGATGGCAGGGCA | CCCCAACTCCCTCTCCCTAA | Start: 3243 |
| Tm | 60.032 | 59.956 | End: 3424 |
| Size | 20 | 20 | 182 |
(T)10aa(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAGAAACACAGGCAGTTGC | TGCCCTGCCATCATCCAATT | Start: 3162 |
| Tm | 59.969 | 60.032 | End: 3262 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | TTTCCCGCGAACGTAAAGGA | TGCCCTGCCATCATCCAATT | Start: 3107 |
| Tm | 59.967 | 60.032 | End: 3262 |
| Size | 20 | 20 | 156 |
| Primer 3 details |
|---|
| Sequence | AATTGGATGATGGCAGGGCA | CCGTGAAGTACTGTGACGCT | Start: 3243 |
| Tm | 60.032 | 60.039 | End: 3490 |
| Size | 20 | 20 | 248 |
| Primer 4 details |
|---|
| Sequence | CCAGAAACACAGGCAGTTGC | CCCCAACTCCCTCTCCCTAA | Start: 3162 |
| Tm | 59.969 | 59.956 | End: 3424 |
| Size | 20 | 20 | 263 |
| Primer 5 details |
|---|
| Sequence | AATTGGATGATGGCAGGGCA | CCCCAACTCCCTCTCCCTAA | Start: 3243 |
| Tm | 60.032 | 59.956 | End: 3424 |
| Size | 20 | 20 | 182 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAGAAACACAGGCAGTTGC | TGCCCTGCCATCATCCAATT | Start: 3162 |
| Tm | 59.969 | 60.032 | End: 3262 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | TTTCCCGCGAACGTAAAGGA | TGCCCTGCCATCATCCAATT | Start: 3107 |
| Tm | 59.967 | 60.032 | End: 3262 |
| Size | 20 | 20 | 156 |
| Primer 3 details |
|---|
| Sequence | AATTGGATGATGGCAGGGCA | CCGTGAAGTACTGTGACGCT | Start: 3243 |
| Tm | 60.032 | 60.039 | End: 3490 |
| Size | 20 | 20 | 248 |
| Primer 4 details |
|---|
| Sequence | CCAGAAACACAGGCAGTTGC | CCCCAACTCCCTCTCCCTAA | Start: 3162 |
| Tm | 59.969 | 59.956 | End: 3424 |
| Size | 20 | 20 | 263 |
| Primer 5 details |
|---|
| Sequence | AATTGGATGATGGCAGGGCA | CCCCAACTCCCTCTCCCTAA | Start: 3243 |
| Tm | 60.032 | 59.956 | End: 3424 |
| Size | 20 | 20 | 182 |
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