ssr_primer
Primer Details for TRINITY_DN63305_c6_g2_i1 |
|---|
| (A)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGATTCACTGCCGCGAAAG | AAAGACGACGATGGCAAGGT | Start: 410 |
| Tm | 59.904 | 59.965 | End: 689 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | CTGATTCACTGCCGCGAAAG | TCTCCCTCCGTCCTCTTTGT | Start: 410 |
| Tm | 59.904 | 59.885 | End: 630 |
| Size | 20 | 20 | 221 |
| Primer 3 details |
|---|
| Sequence | ATAAATGACTGCCGGCTCCC | AAAGACGACGATGGCAAGGT | Start: 547 |
| Tm | 60.179 | 59.965 | End: 689 |
| Size | 20 | 20 | 143 |
| Primer 4 details |
|---|
| Sequence | ATTCACTGCCGCGAAAGTTG | AAAGACGACGATGGCAAGGT | Start: 413 |
| Tm | 59.762 | 59.965 | End: 689 |
| Size | 20 | 20 | 277 |
| Primer 5 details |
|---|
| Sequence | CTGATTCACTGCCGCGAAAG | GGGAGCCGGCAGTCATTTAT | Start: 410 |
| Tm | 59.904 | 60.179 | End: 566 |
| Size | 20 | 20 | 157 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGATTCACTGCCGCGAAAG | AAAGACGACGATGGCAAGGT | Start: 410 |
| Tm | 59.904 | 59.965 | End: 689 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | CTGATTCACTGCCGCGAAAG | TCTCCCTCCGTCCTCTTTGT | Start: 410 |
| Tm | 59.904 | 59.885 | End: 630 |
| Size | 20 | 20 | 221 |
| Primer 3 details |
|---|
| Sequence | ATAAATGACTGCCGGCTCCC | AAAGACGACGATGGCAAGGT | Start: 547 |
| Tm | 60.179 | 59.965 | End: 689 |
| Size | 20 | 20 | 143 |
| Primer 4 details |
|---|
| Sequence | ATTCACTGCCGCGAAAGTTG | AAAGACGACGATGGCAAGGT | Start: 413 |
| Tm | 59.762 | 59.965 | End: 689 |
| Size | 20 | 20 | 277 |
| Primer 5 details |
|---|
| Sequence | CTGATTCACTGCCGCGAAAG | GGGAGCCGGCAGTCATTTAT | Start: 410 |
| Tm | 59.904 | 60.179 | End: 566 |
| Size | 20 | 20 | 157 |
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