ssr_primer
Primer Details for TRINITY_DN63281_c28_g1_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGCGGTTTGATCTTGTGAC | TCCGCAAAAAGGTCCGGATT | Start: 42 |
| Tm | 60.453 | 60.251 | End: 275 |
| Size | 20 | 20 | 234 |
| Primer 2 details |
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| Sequence | GCGCGGTTTGATCTTGTGAC | ATTCCGCAAAAAGGTCCGGA | Start: 42 |
| Tm | 60.453 | 60.251 | End: 277 |
| Size | 20 | 20 | 236 |
| Primer 3 details |
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| Sequence | AGCGCGGTTTGATCTTGTGA | TCCGCAAAAAGGTCCGGATT | Start: 41 |
| Tm | 60.603 | 60.251 | End: 275 |
| Size | 20 | 20 | 235 |
| Primer 4 details |
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| Sequence | AGCGCGGTTTGATCTTGTGA | ATTCCGCAAAAAGGTCCGGA | Start: 41 |
| Tm | 60.603 | 60.251 | End: 277 |
| Size | 20 | 20 | 237 |
| Primer 5 details |
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| Sequence | TCAGTATTTCCGAACCGCCT | CGGGAGAGGGAATCATGAGC | Start: 290 |
| Tm | 59.1 | 59.965 | End: 395 |
| Size | 20 | 20 | 106 |
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