ssr_primer
Primer Details for TRINITY_DN63268_c3_g1_i5 |
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| (AAG)8: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCGGAGCTTTCTCGAAACT | GTCCGTCAAAAGTTCCGGGA | Start: 25 |
| Tm | 60.037 | 60.25 | End: 141 |
| Size | 20 | 20 | 117 |
| Primer 2 details |
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| Sequence | TCCCGGAGCTTTCTCGAAAC | GTCCGTCAAAAGTTCCGGGA | Start: 24 |
| Tm | 60.038 | 60.25 | End: 141 |
| Size | 20 | 20 | 118 |
| Primer 3 details |
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| Sequence | CCCGGAGCTTTCTCGAAACT | TGTCCGTCAAAAGTTCCGGG | Start: 25 |
| Tm | 60.037 | 60.533 | End: 142 |
| Size | 20 | 20 | 118 |
| Primer 4 details |
|---|
| Sequence | TCCCGGAGCTTTCTCGAAAC | TGTCCGTCAAAAGTTCCGGG | Start: 24 |
| Tm | 60.038 | 60.533 | End: 142 |
| Size | 20 | 20 | 119 |
| Primer 5 details |
|---|
| Sequence | CCCGAAACTTCTGACGGTCA | AAAGAATGGTCGTGGGCTCG | Start: 67 |
| Tm | 59.967 | 60.674 | End: 217 |
| Size | 20 | 20 | 151 |
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