ssr_primer
Primer Details for TRINITY_DN63235_c1_g1_i3 |
|---|
| (A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCTTTCTCTCGCTCGCTCG | TGGGTTACTACGGCCACAAC | Start: 1453 |
| Tm | 59.971 | 59.965 | End: 1613 |
| Size | 20 | 20 | 161 |
| Primer 2 details |
|---|
| Sequence | CCCGGCGCCTTATATTGACT | CATGCGAGCACGAATAACCG | Start: 119 |
| Tm | 59.965 | 60.043 | End: 283 |
| Size | 20 | 20 | 165 |
| Primer 3 details |
|---|
| Sequence | CGGTTATTCGTGCTCGCATG | TAACCCCTCACCCATTGTGC | Start: 264 |
| Tm | 60.043 | 59.961 | End: 478 |
| Size | 20 | 20 | 215 |
| Primer 4 details |
|---|
| Sequence | CAACGGTCAACATCGCCATC | CGAGCGAGCGAGAGAAAGAT | Start: 1197 |
| Tm | 59.903 | 59.971 | End: 1472 |
| Size | 20 | 20 | 276 |
| Primer 5 details |
|---|
| Sequence | ATCTTTCTCTCGCTCGCTCG | GACCCCGTAATCCAACCCAG | Start: 1453 |
| Tm | 59.971 | 60.107 | End: 1703 |
| Size | 20 | 20 | 251 |
(A)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCTTTCTCTCGCTCGCTCG | TGGGTTACTACGGCCACAAC | Start: 1453 |
| Tm | 59.971 | 59.965 | End: 1613 |
| Size | 20 | 20 | 161 |
| Primer 2 details |
|---|
| Sequence | CCCGGCGCCTTATATTGACT | CATGCGAGCACGAATAACCG | Start: 119 |
| Tm | 59.965 | 60.043 | End: 283 |
| Size | 20 | 20 | 165 |
| Primer 3 details |
|---|
| Sequence | CGGTTATTCGTGCTCGCATG | TAACCCCTCACCCATTGTGC | Start: 264 |
| Tm | 60.043 | 59.961 | End: 478 |
| Size | 20 | 20 | 215 |
| Primer 4 details |
|---|
| Sequence | CAACGGTCAACATCGCCATC | CGAGCGAGCGAGAGAAAGAT | Start: 1197 |
| Tm | 59.903 | 59.971 | End: 1472 |
| Size | 20 | 20 | 276 |
| Primer 5 details |
|---|
| Sequence | ATCTTTCTCTCGCTCGCTCG | GACCCCGTAATCCAACCCAG | Start: 1453 |
| Tm | 59.971 | 60.107 | End: 1703 |
| Size | 20 | 20 | 251 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCTTTCTCTCGCTCGCTCG | TGGGTTACTACGGCCACAAC | Start: 1453 |
| Tm | 59.971 | 59.965 | End: 1613 |
| Size | 20 | 20 | 161 |
| Primer 2 details |
|---|
| Sequence | CCCGGCGCCTTATATTGACT | CATGCGAGCACGAATAACCG | Start: 119 |
| Tm | 59.965 | 60.043 | End: 283 |
| Size | 20 | 20 | 165 |
| Primer 3 details |
|---|
| Sequence | CGGTTATTCGTGCTCGCATG | TAACCCCTCACCCATTGTGC | Start: 264 |
| Tm | 60.043 | 59.961 | End: 478 |
| Size | 20 | 20 | 215 |
| Primer 4 details |
|---|
| Sequence | CAACGGTCAACATCGCCATC | CGAGCGAGCGAGAGAAAGAT | Start: 1197 |
| Tm | 59.903 | 59.971 | End: 1472 |
| Size | 20 | 20 | 276 |
| Primer 5 details |
|---|
| Sequence | ATCTTTCTCTCGCTCGCTCG | GACCCCGTAATCCAACCCAG | Start: 1453 |
| Tm | 59.971 | 60.107 | End: 1703 |
| Size | 20 | 20 | 251 |
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