ssr_primer
Primer Details for TRINITY_DN63231_c0_g1_i7 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAACCAGCGTCCTTTCTTG | AACGTGTAACTGCTCGGGAG | Start: 540 |
| Tm | 60.04 | 60.039 | End: 765 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | ATTGCTCCACGGGAACTTGT | AAGGACGCTGGTTGCTCTAC | Start: 394 |
| Tm | 59.89 | 60.037 | End: 554 |
| Size | 20 | 20 | 161 |
| Primer 3 details |
|---|
| Sequence | ATTGCTCCACGGGAACTTGT | CAAGAAAGGACGCTGGTTGC | Start: 394 |
| Tm | 59.89 | 60.04 | End: 559 |
| Size | 20 | 20 | 166 |
| Primer 4 details |
|---|
| Sequence | GCCGAATCTCTTGGACGAGT | AACGTGTAACTGCTCGGGAG | Start: 497 |
| Tm | 59.826 | 60.039 | End: 765 |
| Size | 20 | 20 | 269 |
| Primer 5 details |
|---|
| Sequence | ATTGCTCCACGGGAACTTGT | ACTCGTCCAAGAGATTCGGC | Start: 394 |
| Tm | 59.89 | 59.826 | End: 516 |
| Size | 20 | 20 | 123 |
(A)51: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAACCAGCGTCCTTTCTTG | AACGTGTAACTGCTCGGGAG | Start: 540 |
| Tm | 60.04 | 60.039 | End: 765 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | ATTGCTCCACGGGAACTTGT | AAGGACGCTGGTTGCTCTAC | Start: 394 |
| Tm | 59.89 | 60.037 | End: 554 |
| Size | 20 | 20 | 161 |
| Primer 3 details |
|---|
| Sequence | ATTGCTCCACGGGAACTTGT | CAAGAAAGGACGCTGGTTGC | Start: 394 |
| Tm | 59.89 | 60.04 | End: 559 |
| Size | 20 | 20 | 166 |
| Primer 4 details |
|---|
| Sequence | GCCGAATCTCTTGGACGAGT | AACGTGTAACTGCTCGGGAG | Start: 497 |
| Tm | 59.826 | 60.039 | End: 765 |
| Size | 20 | 20 | 269 |
| Primer 5 details |
|---|
| Sequence | ATTGCTCCACGGGAACTTGT | ACTCGTCCAAGAGATTCGGC | Start: 394 |
| Tm | 59.89 | 59.826 | End: 516 |
| Size | 20 | 20 | 123 |
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