Primer Details for TRINITY_DN63227_c12_g1_i1 |
|---|
| (A)9(ATAATAATAATAATTATTAATAAATAGTACACTAAAATTTAAAAAGACGGGGTAAACATTTTAAATCAAGTGCCTATTATACTCTGAAATTGTTTCAGTCAATCATATCAGTCTTATTTTCAGCCTTTTATGTAGAAAAAAAATAATGAATCAGAACACCTT)(AAT)4: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTGGAATTTGGCTTGATGC | GCACTTGATTTAAAATGTTTACCCCG | Start: 2 |
| Tm | 58.995 | 59.91 | End: 142 |
| Size | 21 | 26 | 141 |
| Primer 2 details |
|---|
| Sequence | GCTTGGAATTTGGCTTGATGC | GGCACTTGATTTAAAATGTTTACCCC | Start: 2 |
| Tm | 58.995 | 59.63 | End: 143 |
| Size | 21 | 26 | 142 |
| Primer 3 details |
|---|
| Sequence | GCTTGGAATTTGGCTTGATGCT | GCACTTGATTTAAAATGTTTACCCCG | Start: 2 |
| Tm | 60.354 | 59.91 | End: 142 |
| Size | 22 | 26 | 141 |
| Primer 4 details |
|---|
| Sequence | TGGAATTTGGCTTGATGCTGA | GCACTTGATTTAAAATGTTTACCCCG | Start: 5 |
| Tm | 58.473 | 59.91 | End: 142 |
| Size | 21 | 26 | 138 |
| Primer 5 details |
|---|
| Sequence | TGCTTGGAATTTGGCTTGATGC | GCACTTGATTTAAAATGTTTACCCCG | Start: 1 |
| Tm | 60.613 | 59.91 | End: 142 |
| Size | 22 | 26 | 142 |
