ssr_primer
Primer Details for TRINITY_DN63214_c1_g9_i2 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAGTCGACCGGCTAAAACT | AGTTGCCTTGCTCCCTTGAG | Start: 224 |
| Tm | 59.754 | 60.251 | End: 370 |
| Size | 20 | 20 | 147 |
| Primer 2 details |
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| Sequence | AGGAGTCGACCGGCTAAAAC | AGTTGCCTTGCTCCCTTGAG | Start: 223 |
| Tm | 59.754 | 60.251 | End: 370 |
| Size | 20 | 20 | 148 |
| Primer 3 details |
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| Sequence | AAAAAGAGGAGTCGACCGGC | AGTTGCCTTGCTCCCTTGAG | Start: 217 |
| Tm | 60.321 | 60.251 | End: 370 |
| Size | 20 | 20 | 154 |
| Primer 4 details |
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| Sequence | AGAGGAGTCGACCGGCTAAA | AGTTGCCTTGCTCCCTTGAG | Start: 221 |
| Tm | 60.323 | 60.251 | End: 370 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
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| Sequence | AAGAGGAGTCGACCGGCTAA | AGTTGCCTTGCTCCCTTGAG | Start: 220 |
| Tm | 60.323 | 60.251 | End: 370 |
| Size | 20 | 20 | 151 |
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