ssr_primer
Primer Details for TRINITY_DN63192_c8_g2_i2 |
|---|
| (A)25: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGAATTCCTGTGACCGCCT | GCTGAAGTACCGAGGCTGTT | Start: 371 |
| Tm | 60.036 | 60.037 | End: 617 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
|---|
| Sequence | TGAATTCCTGTGACCGCCTC | GCTGAAGTACCGAGGCTGTT | Start: 372 |
| Tm | 60.036 | 60.037 | End: 617 |
| Size | 20 | 20 | 246 |
| Primer 3 details |
|---|
| Sequence | AATAAACCCGCGACCGTTCT | TCAGCAACTTTCACGTCCGA | Start: 670 |
| Tm | 60.037 | 59.897 | End: 821 |
| Size | 20 | 20 | 152 |
| Primer 4 details |
|---|
| Sequence | AACAGCCTCGGTACTTCAGC | TCAGCAACTTTCACGTCCGA | Start: 598 |
| Tm | 60.037 | 59.897 | End: 821 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | TTGCCCTGAAACCTGAGTCC | AGGCGGTCACAGGAATTCAG | Start: 138 |
| Tm | 59.89 | 60.036 | End: 390 |
| Size | 20 | 20 | 253 |
(A)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGAATTCCTGTGACCGCCT | GCTGAAGTACCGAGGCTGTT | Start: 371 |
| Tm | 60.036 | 60.037 | End: 617 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
|---|
| Sequence | TGAATTCCTGTGACCGCCTC | GCTGAAGTACCGAGGCTGTT | Start: 372 |
| Tm | 60.036 | 60.037 | End: 617 |
| Size | 20 | 20 | 246 |
| Primer 3 details |
|---|
| Sequence | AATAAACCCGCGACCGTTCT | TCAGCAACTTTCACGTCCGA | Start: 670 |
| Tm | 60.037 | 59.897 | End: 821 |
| Size | 20 | 20 | 152 |
| Primer 4 details |
|---|
| Sequence | AACAGCCTCGGTACTTCAGC | TCAGCAACTTTCACGTCCGA | Start: 598 |
| Tm | 60.037 | 59.897 | End: 821 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | TTGCCCTGAAACCTGAGTCC | AGGCGGTCACAGGAATTCAG | Start: 138 |
| Tm | 59.89 | 60.036 | End: 390 |
| Size | 20 | 20 | 253 |
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