ssr_primer
Primer Details for TRINITY_DN63176_c5_g1_i4 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTTCTAGACTCGACGGGT | TCTACGTGGGCCCTTTTGTC | Start: 1856 |
| Tm | 59.963 | 59.964 | End: 1993 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
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| Sequence | CCCTTCACCTTCTTTCCCCC | ACGAGCGTCCAGTTGTTCAT | Start: 1127 |
| Tm | 59.96 | 59.966 | End: 1248 |
| Size | 20 | 20 | 122 |
| Primer 3 details |
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| Sequence | CCCTTCACCTTCTTTCCCCC | GGCAGAAACATCGCAAAGCA | Start: 1127 |
| Tm | 59.96 | 60.039 | End: 1285 |
| Size | 20 | 20 | 159 |
| Primer 4 details |
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| Sequence | CGTGGTTCTAGACTCGACGG | TCTACGTGGGCCCTTTTGTC | Start: 1854 |
| Tm | 59.903 | 59.964 | End: 1993 |
| Size | 20 | 20 | 140 |
| Primer 5 details |
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| Sequence | AAGTAACCGAGGGGAAAGCG | ACTCTGTTGCTGCCAGGTTT | Start: 773 |
| Tm | 60.037 | 60.107 | End: 913 |
| Size | 20 | 20 | 141 |
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