ssr_primer
Primer Details for TRINITY_DN63144_c7_g1_i2 |
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| (T)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACTCCCAACCGCCATACAG | TTGAACGTGTCAGCGAGGAA | Start: 172 |
| Tm | 60.035 | 59.897 | End: 303 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
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| Sequence | AACTCCCAACCGCCATACAG | TGAACGTGTCAGCGAGGAAT | Start: 172 |
| Tm | 60.035 | 59.683 | End: 302 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
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| Sequence | AACTCCCAACCGCCATACAG | ATTGAACGTGTCAGCGAGGA | Start: 172 |
| Tm | 60.035 | 59.683 | End: 304 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
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| Sequence | CCGTCACCGTCCCTGAAAAT | GAGATGCAATGAGTGGGGCT | Start: 530 |
| Tm | 60.321 | 60.107 | End: 667 |
| Size | 20 | 20 | 138 |
| Primer 5 details |
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| Sequence | CCGTCACCGTCCCTGAAAAT | AGCTACGATCGGCGAAGAAC | Start: 530 |
| Tm | 60.321 | 60.249 | End: 689 |
| Size | 20 | 20 | 160 |
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