Primer Details for TRINITY_DN63131_c14_g1_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCAATGTTTAATTCCCGTTCAT | ACTCAACTACTGTACATGTACTGT | Start: 0 |
| Tm | 57.166 | 57.104 | End: 208 |
| Size | 24 | 24 | 209 |
| Primer 2 details |
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| Sequence | TGTTTAATTCCCGTTCATTCTTCA | ACTCAACTACTGTACATGTACTGT | Start: 6 |
| Tm | 57.114 | 57.104 | End: 208 |
| Size | 24 | 24 | 203 |
| Primer 3 details |
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| Sequence | ACTCAATGTTTAATTCCCGTTCAT | CAACTCAACTACTGTACATGTACTGT | Start: 0 |
| Tm | 57.166 | 58.854 | End: 210 |
| Size | 24 | 26 | 211 |
| Primer 4 details |
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| Sequence | ACTCAATGTTTAATTCCCGTTCAT | ACAACTCAACTACTGTACATGTACTG | Start: 0 |
| Tm | 57.166 | 58.854 | End: 211 |
| Size | 24 | 26 | 212 |
| Primer 5 details |
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| Sequence | TGTTTAATTCCCGTTCATTCTTCA | CAACTCAACTACTGTACATGTACTGT | Start: 6 |
| Tm | 57.114 | 58.854 | End: 210 |
| Size | 24 | 26 | 205 |
(A)20gccaataaaccaacaaaaaaaaat(A)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCAATGTTTAATTCCCGTTCAT | ACTCAACTACTGTACATGTACTGT | Start: 0 |
| Tm | 57.166 | 57.104 | End: 208 |
| Size | 24 | 24 | 209 |
| Primer 2 details |
|---|
| Sequence | TGTTTAATTCCCGTTCATTCTTCA | ACTCAACTACTGTACATGTACTGT | Start: 6 |
| Tm | 57.114 | 57.104 | End: 208 |
| Size | 24 | 24 | 203 |
| Primer 3 details |
|---|
| Sequence | ACTCAATGTTTAATTCCCGTTCAT | CAACTCAACTACTGTACATGTACTGT | Start: 0 |
| Tm | 57.166 | 58.854 | End: 210 |
| Size | 24 | 26 | 211 |
| Primer 4 details |
|---|
| Sequence | ACTCAATGTTTAATTCCCGTTCAT | ACAACTCAACTACTGTACATGTACTG | Start: 0 |
| Tm | 57.166 | 58.854 | End: 211 |
| Size | 24 | 26 | 212 |
| Primer 5 details |
|---|
| Sequence | TGTTTAATTCCCGTTCATTCTTCA | CAACTCAACTACTGTACATGTACTGT | Start: 6 |
| Tm | 57.114 | 58.854 | End: 210 |
| Size | 24 | 26 | 205 |
