ssr_primer
Primer Details for TRINITY_DN63126_c1_g1_i1 |
|---|
| (T)10gtttttttttgttttttg(T)13ggcggggcccacctctgaaaatttttgaccagtccgtccctaatgagattcgcaaaattacgaga(G)26: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCTACGGGCCCCAAATGTT | TTAAGGGAGGAGGGGAGAGC | Start: 334 |
| Tm | 60.034 | 60.031 | End: 527 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | TCTGGCAAAATCTGGGGGTC | TTAAGGGAGGAGGGGAGAGC | Start: 272 |
| Tm | 59.96 | 60.031 | End: 527 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
|---|
| Sequence | CGGGCCCGAAATGTTTGTTT | GACCCCCAGATTTTGCCAGA | Start: 50 |
| Tm | 59.967 | 59.96 | End: 291 |
| Size | 20 | 20 | 242 |
| Primer 4 details |
|---|
| Sequence | CTGAACATGCTTACAGCGGC | TTAAGGGAGGAGGGGAGAGC | Start: 248 |
| Tm | 59.902 | 60.031 | End: 527 |
| Size | 20 | 20 | 280 |
| Primer 5 details |
|---|
| Sequence | CTGAACATGCTTACAGCGGC | AACATTTGGGGCCCGTAGAG | Start: 248 |
| Tm | 59.902 | 60.034 | End: 353 |
| Size | 20 | 20 | 106 |
(T)22ggcggggcccgcctctgaaaatttttggccagtccgtccccaatgagattggcaaaattacga(G)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCTACGGGCCCCAAATGTT | TTAAGGGAGGAGGGGAGAGC | Start: 334 |
| Tm | 60.034 | 60.031 | End: 527 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | TCTGGCAAAATCTGGGGGTC | TTAAGGGAGGAGGGGAGAGC | Start: 272 |
| Tm | 59.96 | 60.031 | End: 527 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
|---|
| Sequence | CGGGCCCGAAATGTTTGTTT | GACCCCCAGATTTTGCCAGA | Start: 50 |
| Tm | 59.967 | 59.96 | End: 291 |
| Size | 20 | 20 | 242 |
| Primer 4 details |
|---|
| Sequence | CTGAACATGCTTACAGCGGC | TTAAGGGAGGAGGGGAGAGC | Start: 248 |
| Tm | 59.902 | 60.031 | End: 527 |
| Size | 20 | 20 | 280 |
| Primer 5 details |
|---|
| Sequence | CTGAACATGCTTACAGCGGC | AACATTTGGGGCCCGTAGAG | Start: 248 |
| Tm | 59.902 | 60.034 | End: 353 |
| Size | 20 | 20 | 106 |
(C)21aacc(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCTACGGGCCCCAAATGTT | TTAAGGGAGGAGGGGAGAGC | Start: 334 |
| Tm | 60.034 | 60.031 | End: 527 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | TCTGGCAAAATCTGGGGGTC | TTAAGGGAGGAGGGGAGAGC | Start: 272 |
| Tm | 59.96 | 60.031 | End: 527 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
|---|
| Sequence | CGGGCCCGAAATGTTTGTTT | GACCCCCAGATTTTGCCAGA | Start: 50 |
| Tm | 59.967 | 59.96 | End: 291 |
| Size | 20 | 20 | 242 |
| Primer 4 details |
|---|
| Sequence | CTGAACATGCTTACAGCGGC | TTAAGGGAGGAGGGGAGAGC | Start: 248 |
| Tm | 59.902 | 60.031 | End: 527 |
| Size | 20 | 20 | 280 |
| Primer 5 details |
|---|
| Sequence | CTGAACATGCTTACAGCGGC | AACATTTGGGGCCCGTAGAG | Start: 248 |
| Tm | 59.902 | 60.034 | End: 353 |
| Size | 20 | 20 | 106 |
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