ssr_primer
Primer Details for TRINITY_DN63117_c5_g1_i2 |
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| (T)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAGCGATGGACACAGAGGG | TCCTCCAAGCCCCTCAGTTA | Start: 27 |
| Tm | 60.036 | 59.882 | End: 139 |
| Size | 20 | 20 | 113 |
| Primer 2 details |
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| Sequence | TTTTCCGCGATAGAGCTCCG | CACGGATCCTTACGGCGTAA | Start: 299 |
| Tm | 60.249 | 59.899 | End: 495 |
| Size | 20 | 20 | 197 |
| Primer 3 details |
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| Sequence | TAACTGAGGGGCTTGGAGGA | CGGAGCTCTATCGCGGAAAA | Start: 120 |
| Tm | 59.882 | 60.249 | End: 318 |
| Size | 20 | 20 | 199 |
| Primer 4 details |
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| Sequence | GCGATAGAGCTCCGTGTGAG | CACGGATCCTTACGGCGTAA | Start: 305 |
| Tm | 60.317 | 59.899 | End: 495 |
| Size | 20 | 20 | 191 |
| Primer 5 details |
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| Sequence | TAACTGAGGGGCTTGGAGGA | CTCACACGGAGCTCTATCGC | Start: 120 |
| Tm | 59.882 | 60.317 | End: 324 |
| Size | 20 | 20 | 205 |
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