ssr_primer
Primer Details for TRINITY_DN63101_c5_g1_i7 |
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| (A)46: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACGACGAAAACGGCGATAC | TTTTCATGCGGAGGTACCCC | Start: 2024 |
| Tm | 59.977 | 60.035 | End: 2167 |
| Size | 20 | 20 | 144 |
| Primer 2 details |
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| Sequence | TGTCGGTGAGGGTTTGATGG | TTTTCATGCGGAGGTACCCC | Start: 1987 |
| Tm | 59.964 | 60.035 | End: 2167 |
| Size | 20 | 20 | 181 |
| Primer 3 details |
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| Sequence | GGTGTCGGTGAGGGTTTGAT | TTTTCATGCGGAGGTACCCC | Start: 1985 |
| Tm | 59.964 | 60.035 | End: 2167 |
| Size | 20 | 20 | 183 |
| Primer 4 details |
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| Sequence | TTCTGCGGCAATTTTTCGGG | ATAATGCGGGCGTGTAGGAG | Start: 1015 |
| Tm | 60.039 | 59.966 | End: 1235 |
| Size | 20 | 20 | 221 |
| Primer 5 details |
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| Sequence | TTCTGCGGCAATTTTTCGGG | GTTCGGGCCTGGAAAAGAGA | Start: 1015 |
| Tm | 60.039 | 59.964 | End: 1176 |
| Size | 20 | 20 | 162 |
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