ssr_primer
Primer Details for TRINITY_DN63003_c4_g2_i1 |
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| (A)24: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACAGACGGTAAGGGGCTTT | CGGAATGCGGACGGTATGTA | Start: 498 |
| Tm | 59.964 | 59.969 | End: 681 |
| Size | 20 | 20 | 184 |
| Primer 2 details |
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| Sequence | CACAGACGGTAAGGGGCTTT | TTCAATTGCTCCGGCTGCTA | Start: 498 |
| Tm | 59.964 | 60.036 | End: 727 |
| Size | 20 | 20 | 230 |
| Primer 3 details |
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| Sequence | ATTCCAGGGACGAACGCAAT | ATTCGACAGAGGCGCTATCG | Start: 218 |
| Tm | 60.035 | 60.04 | End: 384 |
| Size | 20 | 20 | 167 |
| Primer 4 details |
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| Sequence | ATTCCAGGGACGAACGCAAT | CGATTCGACAGAGGCGCTAT | Start: 218 |
| Tm | 60.035 | 60.04 | End: 386 |
| Size | 20 | 20 | 169 |
| Primer 5 details |
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| Sequence | ATAGCGCCTCTGTCGAATCG | AAAGCCCCTTACCGTCTGTG | Start: 367 |
| Tm | 60.04 | 59.964 | End: 517 |
| Size | 20 | 20 | 151 |
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