Primer Details for TRINITY_DN62964_c7_g1_i1 | |||
|---|---|---|---|
| (T)14: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACATCCGTTTCAATATCTCCGA | AGCAATGTTTCAAATTTTTGCCGT | Start: 90 |
| Tm | 57.334 | 59.665 | End: 286 |
| Size | 22 | 24 | 197 |
| Primer 2 details | |||
| Sequence | ACATCCGTTTCAATATCTCCGA | GCAATGTTTCAAATTTTTGCCGT | Start: 90 |
| Tm | 57.334 | 58.404 | End: 285 |
| Size | 22 | 23 | 196 |
| Primer 3 details | |||
| Sequence | TCCGTTTCAATATCTCCGATCA | AGCAATGTTTCAAATTTTTGCCGT | Start: 93 |
| Tm | 57.07 | 59.665 | End: 286 |
| Size | 22 | 24 | 194 |
| Primer 4 details | |||
| Sequence | TGAGCAATTTTAAACCTCGAGTGA | AGCAATGTTTCAAATTTTTGCCGT | Start: 34 |
| Tm | 58.939 | 59.665 | End: 286 |
| Size | 24 | 24 | 253 |
| Primer 5 details | |||
| Sequence | TGTACATCCGTTTCAATATCTCCGA | AGCAATGTTTCAAATTTTTGCCGT | Start: 87 |
| Tm | 59.932 | 59.665 | End: 286 |
| Size | 25 | 24 | 200 |
