ssr_primer
Primer Details for TRINITY_DN62953_c3_g1_i5 |
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| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTTGGCCGGGGATGTTTTC | CTACGAGCCCACGGAACTTT | Start: 46 |
| Tm | 60.038 | 60.038 | End: 255 |
| Size | 20 | 20 | 210 |
| Primer 2 details |
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| Sequence | TTTCGGGTCGGGTAATAGCG | CTACGAGCCCACGGAACTTT | Start: 62 |
| Tm | 59.897 | 60.038 | End: 255 |
| Size | 20 | 20 | 194 |
| Primer 3 details |
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| Sequence | CCTGGCATTAAGAGCGGCTA | CTACGAGCCCACGGAACTTT | Start: 123 |
| Tm | 59.893 | 60.038 | End: 255 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
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| Sequence | CTAAGATCCGCCGCCTTTGA | CTACGAGCCCACGGAACTTT | Start: 140 |
| Tm | 60.179 | 60.038 | End: 255 |
| Size | 20 | 20 | 116 |
| Primer 5 details |
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| Sequence | GTTTGGCCGGGGATGTTTTC | TCAAAGGCGGCGGATCTTAG | Start: 46 |
| Tm | 60.038 | 60.179 | End: 159 |
| Size | 20 | 20 | 114 |
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