ssr_primer
Primer Details for TRINITY_DN62917_c4_g1_i2 |
|---|
| (T)28: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCCTGCTGTACTTGTAGCA | CCATACATTGGGCCCCAACT | Start: 1975 |
| Tm | 60.036 | 60.032 | End: 2131 |
| Size | 20 | 20 | 157 |
| Primer 2 details |
|---|
| Sequence | GCCCTGCTGTACTTGTAGCA | ACGATTAGAGGGGTGGACGA | Start: 1975 |
| Tm | 60.036 | 60.034 | End: 2091 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
|---|
| Sequence | GCCTCAAAGAAAGCTTGCCC | CTGGTCTGGTCCAACGACTC | Start: 837 |
| Tm | 60.038 | 60.038 | End: 1009 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | GGCCTCAAAGAAAGCTTGCC | CTGGTCTGGTCCAACGACTC | Start: 836 |
| Tm | 60.038 | 60.038 | End: 1009 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
|---|
| Sequence | TCAGACGATCTTCTGTCGCG | TTCAGACGTAAGGGCGCAAT | Start: 251 |
| Tm | 59.903 | 60.037 | End: 376 |
| Size | 20 | 20 | 126 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCCTGCTGTACTTGTAGCA | CCATACATTGGGCCCCAACT | Start: 1975 |
| Tm | 60.036 | 60.032 | End: 2131 |
| Size | 20 | 20 | 157 |
| Primer 2 details |
|---|
| Sequence | GCCCTGCTGTACTTGTAGCA | ACGATTAGAGGGGTGGACGA | Start: 1975 |
| Tm | 60.036 | 60.034 | End: 2091 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
|---|
| Sequence | GCCTCAAAGAAAGCTTGCCC | CTGGTCTGGTCCAACGACTC | Start: 837 |
| Tm | 60.038 | 60.038 | End: 1009 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | GGCCTCAAAGAAAGCTTGCC | CTGGTCTGGTCCAACGACTC | Start: 836 |
| Tm | 60.038 | 60.038 | End: 1009 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
|---|
| Sequence | TCAGACGATCTTCTGTCGCG | TTCAGACGTAAGGGCGCAAT | Start: 251 |
| Tm | 59.903 | 60.037 | End: 376 |
| Size | 20 | 20 | 126 |
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