Primer Details for TRINITY_DN62853_c3_g7_i1 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGACTAATATTTGTGCTGAAATGGCA | TGTCTGTAGCCTGAATGAGTCT | Start: 81 |
| Tm | 59.848 | 58.565 | End: 207 |
| Size | 26 | 22 | 127 |
| Primer 2 details |
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| Sequence | TGCTTCGAAAAGTGTATCTGTTAACA | TGTCTGTAGCCTGAATGAGTCT | Start: 31 |
| Tm | 59.519 | 58.565 | End: 207 |
| Size | 26 | 22 | 177 |
| Primer 3 details |
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| Sequence | ACTAATATTTGTGCTGAAATGGCA | TGTCTGTAGCCTGAATGAGTCT | Start: 83 |
| Tm | 57.471 | 58.565 | End: 207 |
| Size | 24 | 22 | 125 |
| Primer 4 details |
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| Sequence | TGGCAGCAATATATTGATTCCTTTGA | TGTCTGTAGCCTGAATGAGTCT | Start: 102 |
| Tm | 59.45 | 58.565 | End: 207 |
| Size | 26 | 22 | 106 |
| Primer 5 details |
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| Sequence | TGACTAATATTTGTGCTGAAATGGCA | TTGTCTGTAGCCTGAATGAGTCT | Start: 81 |
| Tm | 59.848 | 59.165 | End: 208 |
| Size | 26 | 23 | 128 |
