ssr_primer
Primer Details for TRINITY_DN62732_c2_g2_i5 |
|---|
| (T)35: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCTCCCATGGCGCATACAG | GCCTCAAGTGGAAACGGAGA | Start: 187 |
| Tm | 59.964 | 59.965 | End: 406 |
| Size | 20 | 20 | 220 |
| Primer 2 details |
|---|
| Sequence | TTGCTGCTATGCTGTGACCA | GCCTCAAGTGGAAACGGAGA | Start: 251 |
| Tm | 59.963 | 59.965 | End: 406 |
| Size | 20 | 20 | 156 |
| Primer 3 details |
|---|
| Sequence | ATCTCCCATGGCGCATACAG | AGCTACCATTTGCAGCACCT | Start: 187 |
| Tm | 59.964 | 59.961 | End: 368 |
| Size | 20 | 20 | 182 |
| Primer 4 details |
|---|
| Sequence | TTGCTGCTATGCTGTGACCA | AGCTACCATTTGCAGCACCT | Start: 251 |
| Tm | 59.963 | 59.961 | End: 368 |
| Size | 20 | 20 | 118 |
| Primer 5 details |
|---|
| Sequence | ATCTCCCATGGCGCATACAG | GTCAATAACGCACGTGAGCC | Start: 187 |
| Tm | 59.964 | 59.905 | End: 439 |
| Size | 20 | 20 | 253 |
(T)10atta(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCTCCCATGGCGCATACAG | GCCTCAAGTGGAAACGGAGA | Start: 187 |
| Tm | 59.964 | 59.965 | End: 406 |
| Size | 20 | 20 | 220 |
| Primer 2 details |
|---|
| Sequence | TTGCTGCTATGCTGTGACCA | GCCTCAAGTGGAAACGGAGA | Start: 251 |
| Tm | 59.963 | 59.965 | End: 406 |
| Size | 20 | 20 | 156 |
| Primer 3 details |
|---|
| Sequence | ATCTCCCATGGCGCATACAG | AGCTACCATTTGCAGCACCT | Start: 187 |
| Tm | 59.964 | 59.961 | End: 368 |
| Size | 20 | 20 | 182 |
| Primer 4 details |
|---|
| Sequence | TTGCTGCTATGCTGTGACCA | AGCTACCATTTGCAGCACCT | Start: 251 |
| Tm | 59.963 | 59.961 | End: 368 |
| Size | 20 | 20 | 118 |
| Primer 5 details |
|---|
| Sequence | ATCTCCCATGGCGCATACAG | GTCAATAACGCACGTGAGCC | Start: 187 |
| Tm | 59.964 | 59.905 | End: 439 |
| Size | 20 | 20 | 253 |
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