Primer Details for TRINITY_DN62686_c5_g3_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCATTGCATTTCCTTGTTGTCT | AGGGAGTGAATAAAGTATGAAGGCA | Start: 75 |
| Tm | 59.414 | 59.808 | End: 175 |
| Size | 24 | 25 | 101 |
| Primer 2 details |
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| Sequence | ACTCATTGCATTTCCTTGTTGTCT | GGGAGTGAATAAAGTATGAAGGCA | Start: 75 |
| Tm | 59.414 | 58.563 | End: 174 |
| Size | 24 | 24 | 100 |
| Primer 3 details |
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| Sequence | ACTCATTGCATTTCCTTGTTGTC | AGGGAGTGAATAAAGTATGAAGGCA | Start: 75 |
| Tm | 58.125 | 59.808 | End: 175 |
| Size | 23 | 25 | 101 |
| Primer 4 details |
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| Sequence | ACTCATTGCATTTCCTTGTTGT | AGGGAGTGAATAAAGTATGAAGGCA | Start: 75 |
| Tm | 57.057 | 59.808 | End: 175 |
| Size | 22 | 25 | 101 |
| Primer 5 details |
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| Sequence | ACTCATTGCATTTCCTTGTTGTCTT | AGGGAGTGAATAAAGTATGAAGGCA | Start: 75 |
| Tm | 59.93 | 59.808 | End: 175 |
| Size | 25 | 25 | 101 |
