ssr_primer
Primer Details for TRINITY_DN62677_c10_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTGGAAGATTCTGCACCC | AGAGAGTCAGAACAGTACTGGC | Start: 2 |
| Tm | 59.186 | 59.177 | End: 101 |
| Size | 20 | 22 | 100 |
| Primer 2 details |
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| Sequence | GCTTGGAAGATTCTGCACCC | AAGAGAGTCAGAACAGTACTGGC | Start: 2 |
| Tm | 59.186 | 59.743 | End: 102 |
| Size | 20 | 23 | 101 |
| Primer 3 details |
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| Sequence | GCTTGGAAGATTCTGCACCC | AGATTTCTTGTCTTCTCGCAGGA | Start: 2 |
| Tm | 59.186 | 59.741 | End: 140 |
| Size | 20 | 23 | 139 |
| Primer 4 details |
|---|
| Sequence | AGCTTGGAAGATTCTGCACCC | GAGAGTCAGAACAGTACTGGCA | Start: 1 |
| Tm | 60.615 | 59.441 | End: 100 |
| Size | 21 | 22 | 100 |
| Primer 5 details |
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| Sequence | CTTGGAAGATTCTGCACCCCT | AAGAGAGTCAGAACAGTACTGGC | Start: 3 |
| Tm | 59.995 | 59.743 | End: 102 |
| Size | 21 | 23 | 100 |
(T)23: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTGGAAGATTCTGCACCC | AGAGAGTCAGAACAGTACTGGC | Start: 2 |
| Tm | 59.186 | 59.177 | End: 101 |
| Size | 20 | 22 | 100 |
| Primer 2 details |
|---|
| Sequence | GCTTGGAAGATTCTGCACCC | AAGAGAGTCAGAACAGTACTGGC | Start: 2 |
| Tm | 59.186 | 59.743 | End: 102 |
| Size | 20 | 23 | 101 |
| Primer 3 details |
|---|
| Sequence | GCTTGGAAGATTCTGCACCC | AGATTTCTTGTCTTCTCGCAGGA | Start: 2 |
| Tm | 59.186 | 59.741 | End: 140 |
| Size | 20 | 23 | 139 |
| Primer 4 details |
|---|
| Sequence | AGCTTGGAAGATTCTGCACCC | GAGAGTCAGAACAGTACTGGCA | Start: 1 |
| Tm | 60.615 | 59.441 | End: 100 |
| Size | 21 | 22 | 100 |
| Primer 5 details |
|---|
| Sequence | CTTGGAAGATTCTGCACCCCT | AAGAGAGTCAGAACAGTACTGGC | Start: 3 |
| Tm | 59.995 | 59.743 | End: 102 |
| Size | 21 | 23 | 100 |
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