ssr_primer
Primer Details for TRINITY_DN62600_c7_g2_i2 |
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| (TTC)6attctttaatc(CTT)6cattctttgttc(CTT)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCCTTTTTCTCGCGCTTCT | TGGCGAAACAAAGTGAAGCG | Start: 1112 |
| Tm | 59.965 | 59.971 | End: 1337 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
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| Sequence | CGCTTCACTTTGTTTCGCCA | AATGAACCACCTCCGCTGAG | Start: 1318 |
| Tm | 59.971 | 60.036 | End: 1577 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
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| Sequence | GTCTCCGCTGTTTACCTGCT | CGCGGTTGAATTAGGATGCG | Start: 1703 |
| Tm | 60.037 | 60.042 | End: 1828 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
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| Sequence | AGTCTCCGCTGTTTACCTGC | CGCGGTTGAATTAGGATGCG | Start: 1702 |
| Tm | 60.037 | 60.042 | End: 1828 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
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| Sequence | TGGGCTCCCCTGTCTTATCA | GCAGGTAAACAGCGGAGACT | Start: 1592 |
| Tm | 59.956 | 60.037 | End: 1721 |
| Size | 20 | 20 | 130 |
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