ssr_primer
Primer Details for TRINITY_DN62574_c9_g1_i6 |
|---|
| (T)27: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTCGGCTCCTCTTCTTCCT | GAAGAAGAGGGGAAAGGGGC | Start: 808 |
| Tm | 59.96 | 60.034 | End: 982 |
| Size | 20 | 20 | 175 |
| Primer 2 details |
|---|
| Sequence | TTTTCCACAGGGTTGCCACT | GGTGAGCACACTGGAGCTAG | Start: 196 |
| Tm | 60.033 | 60.108 | End: 362 |
| Size | 20 | 20 | 167 |
| Primer 3 details |
|---|
| Sequence | AGTCGGCTCCTCTTCTTCCT | AAGGGGCAGAAGAGGAAGGA | Start: 808 |
| Tm | 59.96 | 60.179 | End: 969 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | TTTTCCACAGGGTTGCCACT | TTGGCTCGACTACGGAGTTG | Start: 196 |
| Tm | 60.033 | 59.757 | End: 419 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | TTTTCCACAGGGTTGCCACT | TCCTTTAGGGCGTAGGGGAT | Start: 196 |
| Tm | 60.033 | 59.733 | End: 296 |
| Size | 20 | 20 | 101 |
(T)24aaatcaataccaagctgatgagttcggaatgttcttgtttcttttttatggtggcagaaga(G)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTCGGCTCCTCTTCTTCCT | GAAGAAGAGGGGAAAGGGGC | Start: 808 |
| Tm | 59.96 | 60.034 | End: 982 |
| Size | 20 | 20 | 175 |
| Primer 2 details |
|---|
| Sequence | TTTTCCACAGGGTTGCCACT | GGTGAGCACACTGGAGCTAG | Start: 196 |
| Tm | 60.033 | 60.108 | End: 362 |
| Size | 20 | 20 | 167 |
| Primer 3 details |
|---|
| Sequence | AGTCGGCTCCTCTTCTTCCT | AAGGGGCAGAAGAGGAAGGA | Start: 808 |
| Tm | 59.96 | 60.179 | End: 969 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | TTTTCCACAGGGTTGCCACT | TTGGCTCGACTACGGAGTTG | Start: 196 |
| Tm | 60.033 | 59.757 | End: 419 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | TTTTCCACAGGGTTGCCACT | TCCTTTAGGGCGTAGGGGAT | Start: 196 |
| Tm | 60.033 | 59.733 | End: 296 |
| Size | 20 | 20 | 101 |
(TCT)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTCGGCTCCTCTTCTTCCT | GAAGAAGAGGGGAAAGGGGC | Start: 808 |
| Tm | 59.96 | 60.034 | End: 982 |
| Size | 20 | 20 | 175 |
| Primer 2 details |
|---|
| Sequence | TTTTCCACAGGGTTGCCACT | GGTGAGCACACTGGAGCTAG | Start: 196 |
| Tm | 60.033 | 60.108 | End: 362 |
| Size | 20 | 20 | 167 |
| Primer 3 details |
|---|
| Sequence | AGTCGGCTCCTCTTCTTCCT | AAGGGGCAGAAGAGGAAGGA | Start: 808 |
| Tm | 59.96 | 60.179 | End: 969 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | TTTTCCACAGGGTTGCCACT | TTGGCTCGACTACGGAGTTG | Start: 196 |
| Tm | 60.033 | 59.757 | End: 419 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | TTTTCCACAGGGTTGCCACT | TCCTTTAGGGCGTAGGGGAT | Start: 196 |
| Tm | 60.033 | 59.733 | End: 296 |
| Size | 20 | 20 | 101 |
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