ssr_primer
Primer Details for TRINITY_DN62537_c0_g1_i1 |
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| (T)15ggcgctagagctcgtccaagtcgttttga(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTGGGGAGAACTGCTAGCC | TGAGGCTGAACCATCGTACG | Start: 44 |
| Tm | 59.672 | 59.827 | End: 252 |
| Size | 20 | 20 | 209 |
| Primer 2 details |
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| Sequence | TTTGGGGAGAACTGCTAGCC | TGTCAATGGGAAGCAGTGCA | Start: 44 |
| Tm | 59.672 | 60.179 | End: 197 |
| Size | 20 | 20 | 154 |
| Primer 3 details |
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| Sequence | TTTTCCAGGGTGGGTTCTGC | TAAAGGGGGAGAGGAAGGCA | Start: 966 |
| Tm | 60.469 | 59.88 | End: 1071 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
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| Sequence | TTCATGGAAAGACGGTGGGC | CAACCAAAAGGTGCCTGACG | Start: 800 |
| Tm | 60.608 | 59.969 | End: 1039 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
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| Sequence | CGTACGATGGTTCAGCCTCA | ACACGGTAACCTGGGGAAGA | Start: 233 |
| Tm | 59.827 | 60.472 | End: 501 |
| Size | 20 | 20 | 269 |
(G)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTGGGGAGAACTGCTAGCC | TGAGGCTGAACCATCGTACG | Start: 44 |
| Tm | 59.672 | 59.827 | End: 252 |
| Size | 20 | 20 | 209 |
| Primer 2 details |
|---|
| Sequence | TTTGGGGAGAACTGCTAGCC | TGTCAATGGGAAGCAGTGCA | Start: 44 |
| Tm | 59.672 | 60.179 | End: 197 |
| Size | 20 | 20 | 154 |
| Primer 3 details |
|---|
| Sequence | TTTTCCAGGGTGGGTTCTGC | TAAAGGGGGAGAGGAAGGCA | Start: 966 |
| Tm | 60.469 | 59.88 | End: 1071 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | TTCATGGAAAGACGGTGGGC | CAACCAAAAGGTGCCTGACG | Start: 800 |
| Tm | 60.608 | 59.969 | End: 1039 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
|---|
| Sequence | CGTACGATGGTTCAGCCTCA | ACACGGTAACCTGGGGAAGA | Start: 233 |
| Tm | 59.827 | 60.472 | End: 501 |
| Size | 20 | 20 | 269 |
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