ssr_primer
Primer Details for TRINITY_DN62415_c0_g1_i4 |
|---|
| (GTTT)5(GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATAAACGCCTAAAATGCCCCAAATTATTTTTGAGAGAAGTTTAAAATTTAAACATTTTACTCATCCAAACTCACCGCCCACAAACGCTAAATTCGACCGTGCGAGTTTTAACGAGCGTTCTCTACTTAATTTTTTCCTCACTTTTCCTGTTGGCTGATTGAGGCGAGGGGTCAGGGGTCGAGTTAGTAACGAAGAACTGAATTTTTCGATTTTTCCGACGAAAATAAGAAATTTTCCCCCGAACGGCAATACTGGTGGAAAGTTCATTCATGACAATGTGGCACGATTGCGGAAAACGCGGCCACATTTCTTCCTGTTCCTAAGTCGTGTTAACTACGGGGTATAAAATGCCGGGCAGAATAGGATTTCGGGAAAGGGCAAAGAATGCTCTGATGAATTTTAAGCTCGGAGCAGCACGCTGCAGATAAGTGCCATACAATGTAAAGATAAATTGGATGTTTATACACGTCAGAGAAATGAATGCTTGCCCCAATTTTTCGTGGGCGTCCTGTGCTCGGTAATCATTTTGCTGACGTCTGACTTGAAATGTATGTTCCGCCTTTAAATTTCTTCAACGCCGTTTTAGGTGCTACTGTGACCTTTAAGTTCGAGAGGAGATTGCACAATTTGTTGTATGGCTCAGGCTGACAGAATTTAACGAAAGAAGAGGCTTATTTCGGATATACCTCACTGCTGTATATTATTAAGTGAATATCATTTCAAGCTCAACACCTTACTTTTTCAAATGCAAATTTTTTTTTTACAAAATATCTTGACACATAAAGAAAAATCATATTAATGACATTAAAACATGATAATAT)(T)33: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCGCATAAGACGAACACTG | ATTAGATCAGCCGGTGCGAG | Start: 14 |
| Tm | 59.976 | 59.967 | End: 163 |
| Size | 20 | 20 | 150 |
| Primer 2 details |
|---|
| Sequence | CTCGCACCGGCTGATCTAAT | CCACCCAAACTGAAGTCGGA | Start: 144 |
| Tm | 59.967 | 59.892 | End: 361 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | CTCGCACCGGCTGATCTAAT | TCCACCCAAACTGAAGTCGG | Start: 144 |
| Tm | 59.967 | 59.892 | End: 362 |
| Size | 20 | 20 | 219 |
| Primer 4 details |
|---|
| Sequence | TTTTCCCCCGAACGGCAATA | ATGGCACTTATCTGCAGCGT | Start: 816 |
| Tm | 59.962 | 60.108 | End: 1019 |
| Size | 20 | 20 | 204 |
| Primer 5 details |
|---|
| Sequence | TTTTCCCCCGAACGGCAATA | GCATTCTTTGCCCTTTCCCG | Start: 816 |
| Tm | 59.962 | 60.109 | End: 972 |
| Size | 20 | 20 | 157 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCGCATAAGACGAACACTG | ATTAGATCAGCCGGTGCGAG | Start: 14 |
| Tm | 59.976 | 59.967 | End: 163 |
| Size | 20 | 20 | 150 |
| Primer 2 details |
|---|
| Sequence | CTCGCACCGGCTGATCTAAT | CCACCCAAACTGAAGTCGGA | Start: 144 |
| Tm | 59.967 | 59.892 | End: 361 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | CTCGCACCGGCTGATCTAAT | TCCACCCAAACTGAAGTCGG | Start: 144 |
| Tm | 59.967 | 59.892 | End: 362 |
| Size | 20 | 20 | 219 |
| Primer 4 details |
|---|
| Sequence | TTTTCCCCCGAACGGCAATA | ATGGCACTTATCTGCAGCGT | Start: 816 |
| Tm | 59.962 | 60.108 | End: 1019 |
| Size | 20 | 20 | 204 |
| Primer 5 details |
|---|
| Sequence | TTTTCCCCCGAACGGCAATA | GCATTCTTTGCCCTTTCCCG | Start: 816 |
| Tm | 59.962 | 60.109 | End: 972 |
| Size | 20 | 20 | 157 |
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