ssr_primer
Primer Details for TRINITY_DN62384_c9_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTTCCGCGGGTCAAAATA | ACGCCTTGGGAACTACACAG | Start: 653 |
| Tm | 60.036 | 59.965 | End: 875 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
|---|
| Sequence | GCTCTGGTCTTGCCACTCTT | TATGTGTCGTGTGTCGTGGG | Start: 79 |
| Tm | 59.964 | 60.039 | End: 315 |
| Size | 20 | 20 | 237 |
| Primer 3 details |
|---|
| Sequence | CTGTGTAGTTCCCAAGGCGT | GGGGACATCGTCGTCACATT | Start: 856 |
| Tm | 59.965 | 60.108 | End: 995 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | CGTCGATGCCAAGTGTACCT | ACGCCTTGGGAACTACACAG | Start: 748 |
| Tm | 60.109 | 59.965 | End: 875 |
| Size | 20 | 20 | 128 |
| Primer 5 details |
|---|
| Sequence | TCGTCGATGCCAAGTGTACC | ACGCCTTGGGAACTACACAG | Start: 747 |
| Tm | 60.109 | 59.965 | End: 875 |
| Size | 20 | 20 | 129 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTTCCGCGGGTCAAAATA | ACGCCTTGGGAACTACACAG | Start: 653 |
| Tm | 60.036 | 59.965 | End: 875 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
|---|
| Sequence | GCTCTGGTCTTGCCACTCTT | TATGTGTCGTGTGTCGTGGG | Start: 79 |
| Tm | 59.964 | 60.039 | End: 315 |
| Size | 20 | 20 | 237 |
| Primer 3 details |
|---|
| Sequence | CTGTGTAGTTCCCAAGGCGT | GGGGACATCGTCGTCACATT | Start: 856 |
| Tm | 59.965 | 60.108 | End: 995 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | CGTCGATGCCAAGTGTACCT | ACGCCTTGGGAACTACACAG | Start: 748 |
| Tm | 60.109 | 59.965 | End: 875 |
| Size | 20 | 20 | 128 |
| Primer 5 details |
|---|
| Sequence | TCGTCGATGCCAAGTGTACC | ACGCCTTGGGAACTACACAG | Start: 747 |
| Tm | 60.109 | 59.965 | End: 875 |
| Size | 20 | 20 | 129 |
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