ssr_primer
Primer Details for TRINITY_DN61896_c0_g1_i1 |
|---|
| (A)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCGCGATAAATCAAAGGCG | AATCGGGAAAATGGCAACGC | Start: 1090 |
| Tm | 59.973 | 60.109 | End: 1230 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
|---|
| Sequence | TGCGCGATAAATCAAAGGCG | CAATCCGCACGCCGATAATG | Start: 1090 |
| Tm | 59.973 | 60.111 | End: 1267 |
| Size | 20 | 20 | 178 |
| Primer 3 details |
|---|
| Sequence | AGCTCGGGGAAAAGTTAGGC | CAATCCGCACGCCGATAATG | Start: 1168 |
| Tm | 60.035 | 60.111 | End: 1267 |
| Size | 20 | 20 | 100 |
| Primer 4 details |
|---|
| Sequence | AGCTGCCGGCACAGAAATAT | GCTCGAGCACTTGCCTCTAA | Start: 439 |
| Tm | 60.107 | 60.109 | End: 603 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | ATAGCTGCCGGCACAGAAAT | GCTCGAGCACTTGCCTCTAA | Start: 437 |
| Tm | 60.107 | 60.109 | End: 603 |
| Size | 20 | 20 | 167 |
(A)11tcgggaaaagccgaataatagcgaacgaaatacaaatatgcaagaaaaaagaccccggggtgtggt(G)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCGCGATAAATCAAAGGCG | AATCGGGAAAATGGCAACGC | Start: 1090 |
| Tm | 59.973 | 60.109 | End: 1230 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
|---|
| Sequence | TGCGCGATAAATCAAAGGCG | CAATCCGCACGCCGATAATG | Start: 1090 |
| Tm | 59.973 | 60.111 | End: 1267 |
| Size | 20 | 20 | 178 |
| Primer 3 details |
|---|
| Sequence | AGCTCGGGGAAAAGTTAGGC | CAATCCGCACGCCGATAATG | Start: 1168 |
| Tm | 60.035 | 60.111 | End: 1267 |
| Size | 20 | 20 | 100 |
| Primer 4 details |
|---|
| Sequence | AGCTGCCGGCACAGAAATAT | GCTCGAGCACTTGCCTCTAA | Start: 439 |
| Tm | 60.107 | 60.109 | End: 603 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | ATAGCTGCCGGCACAGAAAT | GCTCGAGCACTTGCCTCTAA | Start: 437 |
| Tm | 60.107 | 60.109 | End: 603 |
| Size | 20 | 20 | 167 |
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