ssr_primer
Primer Details for TRINITY_DN61374_c0_g1_i6 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACTTTCGGCTCTTCGTCGT | ACATGGCTGCAGACCATCAA | Start: 727 |
| Tm | 59.968 | 59.961 | End: 878 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
|---|
| Sequence | AACTTTCGGCTCTTCGTCGT | AACATGGCTGCAGACCATCA | Start: 727 |
| Tm | 59.968 | 59.961 | End: 879 |
| Size | 20 | 20 | 153 |
| Primer 3 details |
|---|
| Sequence | ACACCACCGACCCATGAATC | TCAGTGCCTGCGTTCAGATT | Start: 437 |
| Tm | 60.035 | 59.965 | End: 563 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
|---|
| Sequence | TACTGGGAAAGTTCACGGCC | AACCCTCGCTATTGTGCCAA | Start: 98 |
| Tm | 59.964 | 59.962 | End: 224 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | ACACCACCGACCCATGAATC | GTGGCACCCTCATGGAAAGA | Start: 437 |
| Tm | 60.035 | 59.962 | End: 703 |
| Size | 20 | 20 | 267 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACTTTCGGCTCTTCGTCGT | ACATGGCTGCAGACCATCAA | Start: 727 |
| Tm | 59.968 | 59.961 | End: 878 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
|---|
| Sequence | AACTTTCGGCTCTTCGTCGT | AACATGGCTGCAGACCATCA | Start: 727 |
| Tm | 59.968 | 59.961 | End: 879 |
| Size | 20 | 20 | 153 |
| Primer 3 details |
|---|
| Sequence | ACACCACCGACCCATGAATC | TCAGTGCCTGCGTTCAGATT | Start: 437 |
| Tm | 60.035 | 59.965 | End: 563 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
|---|
| Sequence | TACTGGGAAAGTTCACGGCC | AACCCTCGCTATTGTGCCAA | Start: 98 |
| Tm | 59.964 | 59.962 | End: 224 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | ACACCACCGACCCATGAATC | GTGGCACCCTCATGGAAAGA | Start: 437 |
| Tm | 60.035 | 59.962 | End: 703 |
| Size | 20 | 20 | 267 |
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