ssr_primer
Primer Details for TRINITY_DN60916_c0_g1_i1 |
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| (A)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCTTGGAGGTAGGAGAGGA | AGATGGCAGACGCTGAACAA | Start: 180 |
| Tm | 60.032 | 59.965 | End: 340 |
| Size | 20 | 20 | 161 |
| Primer 2 details |
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| Sequence | GGCTTGGAGGTAGGAGAGGA | AAGATGGCAGACGCTGAACA | Start: 180 |
| Tm | 60.032 | 59.965 | End: 341 |
| Size | 20 | 20 | 162 |
| Primer 3 details |
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| Sequence | CACTGTGCACGAGGGTGTAT | TCCTCTCCTACCTCCAAGCC | Start: 100 |
| Tm | 60.037 | 60.032 | End: 199 |
| Size | 20 | 20 | 100 |
| Primer 4 details |
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| Sequence | TGTTCAGCGTCTGCCATCTT | GACTCCGCTTCAGGGTTTCA | Start: 322 |
| Tm | 59.965 | 59.965 | End: 469 |
| Size | 20 | 20 | 148 |
| Primer 5 details |
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| Sequence | TGTTCAGCGTCTGCCATCTT | TGACTCCGCTTCAGGGTTTC | Start: 322 |
| Tm | 59.965 | 59.965 | End: 470 |
| Size | 20 | 20 | 149 |
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