ssr_primer
Primer Details for TRINITY_DN60529_c0_g2_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTAACGCACACTGGAGCAA | TGTCAAGGGGTGTTTCTGGG | Start: 223 |
| Tm | 59.894 | 59.815 | End: 390 |
| Size | 20 | 20 | 168 |
| Primer 2 details |
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| Sequence | TTTCCATTGGCGTTTCGAGC | TTGCTCCAGTGTGCGTTAGT | Start: 112 |
| Tm | 59.76 | 59.894 | End: 242 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
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| Sequence | TTTCCATTGGCGTTTCGAGC | TGTCAAGGGGTGTTTCTGGG | Start: 112 |
| Tm | 59.76 | 59.815 | End: 390 |
| Size | 20 | 20 | 279 |
| Primer 4 details |
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| Sequence | GAATTCCCCCAGCGCACTAA | TGTCAAGGGGTGTTTCTGGG | Start: 208 |
| Tm | 60.394 | 59.815 | End: 390 |
| Size | 20 | 20 | 183 |
| Primer 5 details |
|---|
| Sequence | ACTAACGCACACTGGAGCAA | GTCAAGGGGTGTTTCTGGGA | Start: 223 |
| Tm | 59.894 | 59.523 | End: 389 |
| Size | 20 | 20 | 167 |
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